sprague-dawley rat造句
例句与造句
- Experiments were performed on adult male sprague - dawley rats weighing 230 - 270g
本实验采用健康成年雄性sd大鼠，体重2509士209 。 - Enkephalin ( enk ) is one of endogenous opiate like substance and could induce psychological euphoria and modulate function of learning and memory . however there has no report available about the role of enk in acquisition of sprague - dawley rats as materials
脑啡肽( enk )是内源性阿片样物质的一种，可参与情绪反应调节，引起精神欣快感，并对学习记忆具有调制作用。 - Methods : male sprague - dawley rats were decapitated . the thoracic aorta was removed and washed in cold krebs - henseleit solution , then cut into 2 - 3 mm in length . the endothelium of some rings was removed by rubbing with a metal thread
取雄性sprague - dawley大鼠的胸主动脉条上段，置于4的k - h液中，将血管剪成2 3mm长的主动脉环，部分血管环先用机械法去除血管内皮，迅速悬挂至离体灌流装置中。 - In this experiment , radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process . methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0 . 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al . thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum , and added to 50ml cell culture flasks
方法：胰岛的分离参照王玲等的方法，每次实验取新生3 - 5天sd大鼠，无菌条件下剖腹取出胰腺，剪切为0 . 5mm ~ 3的组织块， v型胶原酶消化30min后，离心洗涤，悬浮于完全培养基，接种入50ml培养瓶，于5 co _ 2 、 95空气条件下培养20h ，转板纯化，接种于96孔培养板培养24h ，按实验要求进行实验。 - Primary culture of rat preadipocyte were prepared from the epididymal , inguinal and perirenal the fat pads of male normal , healthy , 15 - 20 days sprague - dawley rats . the preadipocyte grew better under the condition of 37 , 95 % humidity , 5 % co2 , ph 7 . 0 - 7 . 2 , centrifuged at 1000r / min , m199medium , and 10 % fetal bo vine serum , seeded at a density of 4 l04 , 5 l04 , / cm2 . oil red o staining was the special method to distinguish adipocyte from other cells , gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop , size and the position of the nucleolus of the staining fat cell
经过多次实验，确定本实验室大鼠前体脂肪细胞的最佳培养条件是：温度为37 ，湿度为95 ， co _ 2浓度为5 ， ph值为7 . 0 7 . 2 ，离心力为1000r / min ，培养基为m _ ( 199 )培养基，胎牛血清浓度为10 ，合适细胞接种密度为4 10 ~ 4 、 5 10 ~ 4个/ cm ~ 2 ，染色结果表明：油红o染色是鉴定脂肪细胞的特异方法， gimsa和he染色可根据不同区域染色程度、着色差别判断细胞核的位置及脂滴大小、多少，观察大鼠前体脂肪细胞分化过程中的形态变化，进而确定脂肪细胞的分化阶段。 - It's difficult to find sprague-dawley rat in a sentence. 用sprague-dawley rat造句挺难的
